crest m 15 Search Results


anti crest  (Antibodies Inc)
96
Antibodies Inc anti crest
Anti Crest, supplied by Antibodies Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ilium xylazil (xylazine 15 mg/kg, i.p  (Troy Laboratories)
90
Troy Laboratories ilium xylazil (xylazine 15 mg/kg, i.p
Ilium Xylazil (Xylazine 15 Mg/Kg, I.P, supplied by Troy Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human anti-crest  (Antibodies-Online Inc)
90
Antibodies-Online Inc human anti-crest
Tau loss alters genetic integrity of rDNA and CDA −/− cells. a , b Representative immunofluorescence ( IF ) z ‐projection images showing paired Tau foci ( green ) linked by PICH-positive UFBs in BS ( a ) and HeLa ( b ) anaphase cells. Scale bar : 5 µm. DNA was visualized by DAPI staining ( white ). Centromeres were stained with <t>CREST</t> serum ( blue ) and UFBs were stained with PICH antibody ( red ). In the enlarged images, Tau foci at the extremities of UFBs are indicated by yellow arrows. c Mean number of total and Tau-positive UFBs per anaphase cell. d , f Mean number of R-UFBs per anaphase cell in untreated cell lines. e Relative number of R-UFBs per anaphase cell in BS-Ctrl (BLM) and BS-BLM cells left untreated or treated with 100 µM tetrahydrouridine ( THU ) for 2 × 48 h or 1 mM of deoxycytidine ( dC ) for 10 h. g Mean number of R-UFBs per anaphase cell in BS-Ctrl (BLM) and BS-BLM cells left untreated or treated with 0.4 µM aphidicolin ( APH ) or 1 µM ICRF-159 for 24 h. The results were normalized against those for control conditions (mock), which were set to 1. h HeLa-Ctrl (Tau) and HeLa-shTau cells were first transfected with either non-targeting or CDA siRNA. After 48 h, cells were again transfected with the indicated siRNAs. Three days after the second round of transfection, cells were collected for western blotting, with GAPDH as the loading control ( h ), or were immunostained with antibody against γ-H2AX or 53BP1 ( i , j ). At least 1500 cells were acquired by wide-field microscopy, and γ-H2AX ( i ) or 53BP1 ( j ) foci were counted. The number of cells ( n ) for each condition is indicated. k , l Cells were immunostained <t>with</t> <t>antibodies</t> against PICH and UBTF, and the mean numbers of total and rDNA-associated UFBs per anaphase cell were monitored. For IF experiments, DNA was counterstained with DAPI. For UFB counting, at least 200 cells were acquired by wide-field microscopy. For all data bars, error bars represent ± SD from at least three independent experiments. The significance of differences was assessed in two-tailed unpaired Student’s t -tests. *** P < 0.0005, ** P < 0.005, * P < 0.05, NS not significant
Human Anti Crest, supplied by Antibodies-Online Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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α-ss18l1 (crest) (m-15) antibody  (Santa Cruz Biotechnology)
90
Santa Cruz Biotechnology α-ss18l1 (crest) (m-15) antibody
Tau loss alters genetic integrity of rDNA and CDA −/− cells. a , b Representative immunofluorescence ( IF ) z ‐projection images showing paired Tau foci ( green ) linked by PICH-positive UFBs in BS ( a ) and HeLa ( b ) anaphase cells. Scale bar : 5 µm. DNA was visualized by DAPI staining ( white ). Centromeres were stained with <t>CREST</t> serum ( blue ) and UFBs were stained with PICH antibody ( red ). In the enlarged images, Tau foci at the extremities of UFBs are indicated by yellow arrows. c Mean number of total and Tau-positive UFBs per anaphase cell. d , f Mean number of R-UFBs per anaphase cell in untreated cell lines. e Relative number of R-UFBs per anaphase cell in BS-Ctrl (BLM) and BS-BLM cells left untreated or treated with 100 µM tetrahydrouridine ( THU ) for 2 × 48 h or 1 mM of deoxycytidine ( dC ) for 10 h. g Mean number of R-UFBs per anaphase cell in BS-Ctrl (BLM) and BS-BLM cells left untreated or treated with 0.4 µM aphidicolin ( APH ) or 1 µM ICRF-159 for 24 h. The results were normalized against those for control conditions (mock), which were set to 1. h HeLa-Ctrl (Tau) and HeLa-shTau cells were first transfected with either non-targeting or CDA siRNA. After 48 h, cells were again transfected with the indicated siRNAs. Three days after the second round of transfection, cells were collected for western blotting, with GAPDH as the loading control ( h ), or were immunostained with antibody against γ-H2AX or 53BP1 ( i , j ). At least 1500 cells were acquired by wide-field microscopy, and γ-H2AX ( i ) or 53BP1 ( j ) foci were counted. The number of cells ( n ) for each condition is indicated. k , l Cells were immunostained <t>with</t> <t>antibodies</t> against PICH and UBTF, and the mean numbers of total and rDNA-associated UFBs per anaphase cell were monitored. For IF experiments, DNA was counterstained with DAPI. For UFB counting, at least 200 cells were acquired by wide-field microscopy. For all data bars, error bars represent ± SD from at least three independent experiments. The significance of differences was assessed in two-tailed unpaired Student’s t -tests. *** P < 0.0005, ** P < 0.005, * P < 0.05, NS not significant
α Ss18l1 (Crest) (M 15) Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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autoimmune crest serum 15-235  (Antibodies Inc)
90
Antibodies Inc autoimmune crest serum 15-235
Tau loss alters genetic integrity of rDNA and CDA −/− cells. a , b Representative immunofluorescence ( IF ) z ‐projection images showing paired Tau foci ( green ) linked by PICH-positive UFBs in BS ( a ) and HeLa ( b ) anaphase cells. Scale bar : 5 µm. DNA was visualized by DAPI staining ( white ). Centromeres were stained with <t>CREST</t> serum ( blue ) and UFBs were stained with PICH antibody ( red ). In the enlarged images, Tau foci at the extremities of UFBs are indicated by yellow arrows. c Mean number of total and Tau-positive UFBs per anaphase cell. d , f Mean number of R-UFBs per anaphase cell in untreated cell lines. e Relative number of R-UFBs per anaphase cell in BS-Ctrl (BLM) and BS-BLM cells left untreated or treated with 100 µM tetrahydrouridine ( THU ) for 2 × 48 h or 1 mM of deoxycytidine ( dC ) for 10 h. g Mean number of R-UFBs per anaphase cell in BS-Ctrl (BLM) and BS-BLM cells left untreated or treated with 0.4 µM aphidicolin ( APH ) or 1 µM ICRF-159 for 24 h. The results were normalized against those for control conditions (mock), which were set to 1. h HeLa-Ctrl (Tau) and HeLa-shTau cells were first transfected with either non-targeting or CDA siRNA. After 48 h, cells were again transfected with the indicated siRNAs. Three days after the second round of transfection, cells were collected for western blotting, with GAPDH as the loading control ( h ), or were immunostained with antibody against γ-H2AX or 53BP1 ( i , j ). At least 1500 cells were acquired by wide-field microscopy, and γ-H2AX ( i ) or 53BP1 ( j ) foci were counted. The number of cells ( n ) for each condition is indicated. k , l Cells were immunostained <t>with</t> <t>antibodies</t> against PICH and UBTF, and the mean numbers of total and rDNA-associated UFBs per anaphase cell were monitored. For IF experiments, DNA was counterstained with DAPI. For UFB counting, at least 200 cells were acquired by wide-field microscopy. For all data bars, error bars represent ± SD from at least three independent experiments. The significance of differences was assessed in two-tailed unpaired Student’s t -tests. *** P < 0.0005, ** P < 0.005, * P < 0.05, NS not significant
Autoimmune Crest Serum 15 235, supplied by Antibodies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human polyclonal anti-crest/aca antibody  (Antibodies Inc)
90
Antibodies Inc human polyclonal anti-crest/aca antibody
Tau loss alters genetic integrity of rDNA and CDA −/− cells. a , b Representative immunofluorescence ( IF ) z ‐projection images showing paired Tau foci ( green ) linked by PICH-positive UFBs in BS ( a ) and HeLa ( b ) anaphase cells. Scale bar : 5 µm. DNA was visualized by DAPI staining ( white ). Centromeres were stained with <t>CREST</t> serum ( blue ) and UFBs were stained with PICH antibody ( red ). In the enlarged images, Tau foci at the extremities of UFBs are indicated by yellow arrows. c Mean number of total and Tau-positive UFBs per anaphase cell. d , f Mean number of R-UFBs per anaphase cell in untreated cell lines. e Relative number of R-UFBs per anaphase cell in BS-Ctrl (BLM) and BS-BLM cells left untreated or treated with 100 µM tetrahydrouridine ( THU ) for 2 × 48 h or 1 mM of deoxycytidine ( dC ) for 10 h. g Mean number of R-UFBs per anaphase cell in BS-Ctrl (BLM) and BS-BLM cells left untreated or treated with 0.4 µM aphidicolin ( APH ) or 1 µM ICRF-159 for 24 h. The results were normalized against those for control conditions (mock), which were set to 1. h HeLa-Ctrl (Tau) and HeLa-shTau cells were first transfected with either non-targeting or CDA siRNA. After 48 h, cells were again transfected with the indicated siRNAs. Three days after the second round of transfection, cells were collected for western blotting, with GAPDH as the loading control ( h ), or were immunostained with antibody against γ-H2AX or 53BP1 ( i , j ). At least 1500 cells were acquired by wide-field microscopy, and γ-H2AX ( i ) or 53BP1 ( j ) foci were counted. The number of cells ( n ) for each condition is indicated. k , l Cells were immunostained <t>with</t> <t>antibodies</t> against PICH and UBTF, and the mean numbers of total and rDNA-associated UFBs per anaphase cell were monitored. For IF experiments, DNA was counterstained with DAPI. For UFB counting, at least 200 cells were acquired by wide-field microscopy. For all data bars, error bars represent ± SD from at least three independent experiments. The significance of differences was assessed in two-tailed unpaired Student’s t -tests. *** P < 0.0005, ** P < 0.005, * P < 0.05, NS not significant
Human Polyclonal Anti Crest/Aca Antibody, supplied by Antibodies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ss18l1  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology ss18l1
Tau loss alters genetic integrity of rDNA and CDA −/− cells. a , b Representative immunofluorescence ( IF ) z ‐projection images showing paired Tau foci ( green ) linked by PICH-positive UFBs in BS ( a ) and HeLa ( b ) anaphase cells. Scale bar : 5 µm. DNA was visualized by DAPI staining ( white ). Centromeres were stained with <t>CREST</t> serum ( blue ) and UFBs were stained with PICH antibody ( red ). In the enlarged images, Tau foci at the extremities of UFBs are indicated by yellow arrows. c Mean number of total and Tau-positive UFBs per anaphase cell. d , f Mean number of R-UFBs per anaphase cell in untreated cell lines. e Relative number of R-UFBs per anaphase cell in BS-Ctrl (BLM) and BS-BLM cells left untreated or treated with 100 µM tetrahydrouridine ( THU ) for 2 × 48 h or 1 mM of deoxycytidine ( dC ) for 10 h. g Mean number of R-UFBs per anaphase cell in BS-Ctrl (BLM) and BS-BLM cells left untreated or treated with 0.4 µM aphidicolin ( APH ) or 1 µM ICRF-159 for 24 h. The results were normalized against those for control conditions (mock), which were set to 1. h HeLa-Ctrl (Tau) and HeLa-shTau cells were first transfected with either non-targeting or CDA siRNA. After 48 h, cells were again transfected with the indicated siRNAs. Three days after the second round of transfection, cells were collected for western blotting, with GAPDH as the loading control ( h ), or were immunostained with antibody against γ-H2AX or 53BP1 ( i , j ). At least 1500 cells were acquired by wide-field microscopy, and γ-H2AX ( i ) or 53BP1 ( j ) foci were counted. The number of cells ( n ) for each condition is indicated. k , l Cells were immunostained <t>with</t> <t>antibodies</t> against PICH and UBTF, and the mean numbers of total and rDNA-associated UFBs per anaphase cell were monitored. For IF experiments, DNA was counterstained with DAPI. For UFB counting, at least 200 cells were acquired by wide-field microscopy. For all data bars, error bars represent ± SD from at least three independent experiments. The significance of differences was assessed in two-tailed unpaired Student’s t -tests. *** P < 0.0005, ** P < 0.005, * P < 0.05, NS not significant
Ss18l1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1× non-essential amino acids  (Thermo Fisher)
90
Thermo Fisher 1× non-essential amino acids
Tau loss alters genetic integrity of rDNA and CDA −/− cells. a , b Representative immunofluorescence ( IF ) z ‐projection images showing paired Tau foci ( green ) linked by PICH-positive UFBs in BS ( a ) and HeLa ( b ) anaphase cells. Scale bar : 5 µm. DNA was visualized by DAPI staining ( white ). Centromeres were stained with <t>CREST</t> serum ( blue ) and UFBs were stained with PICH antibody ( red ). In the enlarged images, Tau foci at the extremities of UFBs are indicated by yellow arrows. c Mean number of total and Tau-positive UFBs per anaphase cell. d , f Mean number of R-UFBs per anaphase cell in untreated cell lines. e Relative number of R-UFBs per anaphase cell in BS-Ctrl (BLM) and BS-BLM cells left untreated or treated with 100 µM tetrahydrouridine ( THU ) for 2 × 48 h or 1 mM of deoxycytidine ( dC ) for 10 h. g Mean number of R-UFBs per anaphase cell in BS-Ctrl (BLM) and BS-BLM cells left untreated or treated with 0.4 µM aphidicolin ( APH ) or 1 µM ICRF-159 for 24 h. The results were normalized against those for control conditions (mock), which were set to 1. h HeLa-Ctrl (Tau) and HeLa-shTau cells were first transfected with either non-targeting or CDA siRNA. After 48 h, cells were again transfected with the indicated siRNAs. Three days after the second round of transfection, cells were collected for western blotting, with GAPDH as the loading control ( h ), or were immunostained with antibody against γ-H2AX or 53BP1 ( i , j ). At least 1500 cells were acquired by wide-field microscopy, and γ-H2AX ( i ) or 53BP1 ( j ) foci were counted. The number of cells ( n ) for each condition is indicated. k , l Cells were immunostained <t>with</t> <t>antibodies</t> against PICH and UBTF, and the mean numbers of total and rDNA-associated UFBs per anaphase cell were monitored. For IF experiments, DNA was counterstained with DAPI. For UFB counting, at least 200 cells were acquired by wide-field microscopy. For all data bars, error bars represent ± SD from at least three independent experiments. The significance of differences was assessed in two-tailed unpaired Student’s t -tests. *** P < 0.0005, ** P < 0.005, * P < 0.05, NS not significant
1× Non Essential Amino Acids, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti centromere serum  (Biosynth Carbosynth)
94
Biosynth Carbosynth anti centromere serum
Tau loss alters genetic integrity of rDNA and CDA −/− cells. a , b Representative immunofluorescence ( IF ) z ‐projection images showing paired Tau foci ( green ) linked by PICH-positive UFBs in BS ( a ) and HeLa ( b ) anaphase cells. Scale bar : 5 µm. DNA was visualized by DAPI staining ( white ). Centromeres were stained with <t>CREST</t> serum ( blue ) and UFBs were stained with PICH antibody ( red ). In the enlarged images, Tau foci at the extremities of UFBs are indicated by yellow arrows. c Mean number of total and Tau-positive UFBs per anaphase cell. d , f Mean number of R-UFBs per anaphase cell in untreated cell lines. e Relative number of R-UFBs per anaphase cell in BS-Ctrl (BLM) and BS-BLM cells left untreated or treated with 100 µM tetrahydrouridine ( THU ) for 2 × 48 h or 1 mM of deoxycytidine ( dC ) for 10 h. g Mean number of R-UFBs per anaphase cell in BS-Ctrl (BLM) and BS-BLM cells left untreated or treated with 0.4 µM aphidicolin ( APH ) or 1 µM ICRF-159 for 24 h. The results were normalized against those for control conditions (mock), which were set to 1. h HeLa-Ctrl (Tau) and HeLa-shTau cells were first transfected with either non-targeting or CDA siRNA. After 48 h, cells were again transfected with the indicated siRNAs. Three days after the second round of transfection, cells were collected for western blotting, with GAPDH as the loading control ( h ), or were immunostained with antibody against γ-H2AX or 53BP1 ( i , j ). At least 1500 cells were acquired by wide-field microscopy, and γ-H2AX ( i ) or 53BP1 ( j ) foci were counted. The number of cells ( n ) for each condition is indicated. k , l Cells were immunostained <t>with</t> <t>antibodies</t> against PICH and UBTF, and the mean numbers of total and rDNA-associated UFBs per anaphase cell were monitored. For IF experiments, DNA was counterstained with DAPI. For UFB counting, at least 200 cells were acquired by wide-field microscopy. For all data bars, error bars represent ± SD from at least three independent experiments. The significance of differences was assessed in two-tailed unpaired Student’s t -tests. *** P < 0.0005, ** P < 0.005, * P < 0.05, NS not significant
Anti Centromere Serum, supplied by Biosynth Carbosynth, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human crest antibody  (Antibodies Inc)
90
Antibodies Inc human crest antibody
Increased KIF11 causes PCM fragmentation and unaligned and lagging chromosomes, which give rise to increased numbers of micronuclei. (A) Images of EV CRISPRa (top) and KIF11 CRISPRa (bottom) cells showing dispersed pericentrin and erroneous chromosome congression upon activation. Left to right: <t>DAPI,</t> <t>α-tubulin,</t> <t>CREST,</t> pericentrin and composite images. Yellow arrowheads mark unaligned chromosomes and extra pericentrin foci. (B) Percentage of metaphase cells with more than two pericentrin foci in EV CRISPRa and KIF11 CRISPRa cells (Fisher's exact test). (C) Percentage of metaphase cells with unaligned chromosomes in EV CRISPRa and KIF11 CRISPRa cells (Fisher's exact test). (D) Percentage of metaphase cells with unaligned chromosomes, grouped by pericentrin foci count of two (red) or greater than two (blue) in EV CRISPRa and KIF11 CRISPRa cells (Fisher's exact test). For B–D, n =315 and 324 cells for EV CRISPRa and KIF11 CRISPRa , respectively, three replicates. (E) Images of EV CRISPRa (top) and KIF11 CRISPRa (bottom) cells showing highly dispersed pericentrin and erroneous chromosome segregation in activated cells with more than two pericentrin foci. Left to right: DAPI, α-tubulin, CREST, pericentrin and composite images. Yellow arrowheads mark lagging chromosomes and extra pericentrin foci. (F) Percentage of anaphase cells with more than two pericentrin foci in EV CRISPRa and KIF11 CRISPRa cells (Fisher's exact test, n =330 and 317 cells for EV CRISPRa and KIF11 CRISPRa , respectively, three replicates). (G,H) Percentage of anaphase cells with CREST-positive (centric) lagging chromosomes (G) and CREST-negative (acentric) lagging chromosomes (H) in EV CRISPRa and KIF11 CRISPRa cells (Fisher's exact test). (I) Quantification (left) of acentric and centric micronuclei in EV CRISPRa and KIF11 CRISPRa cells (Fisher's exact test) with example composite images (right) of nuclei stained with DAPI and centromeres stained with CREST. The lower panels are magnified views of micronuclei. Data show the mean±s.e.m. * P <0.05; *** P <0.0005. Scale bars: 10 μm (A); 12 μm (E); 6 μm (I, top); 2 μm (I, bottom).
Human Crest Antibody, supplied by Antibodies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ilium ketamil  (Troy Laboratories)
90
Troy Laboratories ilium ketamil
Increased KIF11 causes PCM fragmentation and unaligned and lagging chromosomes, which give rise to increased numbers of micronuclei. (A) Images of EV CRISPRa (top) and KIF11 CRISPRa (bottom) cells showing dispersed pericentrin and erroneous chromosome congression upon activation. Left to right: <t>DAPI,</t> <t>α-tubulin,</t> <t>CREST,</t> pericentrin and composite images. Yellow arrowheads mark unaligned chromosomes and extra pericentrin foci. (B) Percentage of metaphase cells with more than two pericentrin foci in EV CRISPRa and KIF11 CRISPRa cells (Fisher's exact test). (C) Percentage of metaphase cells with unaligned chromosomes in EV CRISPRa and KIF11 CRISPRa cells (Fisher's exact test). (D) Percentage of metaphase cells with unaligned chromosomes, grouped by pericentrin foci count of two (red) or greater than two (blue) in EV CRISPRa and KIF11 CRISPRa cells (Fisher's exact test). For B–D, n =315 and 324 cells for EV CRISPRa and KIF11 CRISPRa , respectively, three replicates. (E) Images of EV CRISPRa (top) and KIF11 CRISPRa (bottom) cells showing highly dispersed pericentrin and erroneous chromosome segregation in activated cells with more than two pericentrin foci. Left to right: DAPI, α-tubulin, CREST, pericentrin and composite images. Yellow arrowheads mark lagging chromosomes and extra pericentrin foci. (F) Percentage of anaphase cells with more than two pericentrin foci in EV CRISPRa and KIF11 CRISPRa cells (Fisher's exact test, n =330 and 317 cells for EV CRISPRa and KIF11 CRISPRa , respectively, three replicates). (G,H) Percentage of anaphase cells with CREST-positive (centric) lagging chromosomes (G) and CREST-negative (acentric) lagging chromosomes (H) in EV CRISPRa and KIF11 CRISPRa cells (Fisher's exact test). (I) Quantification (left) of acentric and centric micronuclei in EV CRISPRa and KIF11 CRISPRa cells (Fisher's exact test) with example composite images (right) of nuclei stained with DAPI and centromeres stained with CREST. The lower panels are magnified views of micronuclei. Data show the mean±s.e.m. * P <0.05; *** P <0.0005. Scale bars: 10 μm (A); 12 μm (E); 6 μm (I, top); 2 μm (I, bottom).
Ilium Ketamil, supplied by Troy Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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crest autoimmune serum antibody  (Antibodies Inc)
90
Antibodies Inc crest autoimmune serum antibody
Increased KIF11 causes PCM fragmentation and unaligned and lagging chromosomes, which give rise to increased numbers of micronuclei. (A) Images of EV CRISPRa (top) and KIF11 CRISPRa (bottom) cells showing dispersed pericentrin and erroneous chromosome congression upon activation. Left to right: <t>DAPI,</t> <t>α-tubulin,</t> <t>CREST,</t> pericentrin and composite images. Yellow arrowheads mark unaligned chromosomes and extra pericentrin foci. (B) Percentage of metaphase cells with more than two pericentrin foci in EV CRISPRa and KIF11 CRISPRa cells (Fisher's exact test). (C) Percentage of metaphase cells with unaligned chromosomes in EV CRISPRa and KIF11 CRISPRa cells (Fisher's exact test). (D) Percentage of metaphase cells with unaligned chromosomes, grouped by pericentrin foci count of two (red) or greater than two (blue) in EV CRISPRa and KIF11 CRISPRa cells (Fisher's exact test). For B–D, n =315 and 324 cells for EV CRISPRa and KIF11 CRISPRa , respectively, three replicates. (E) Images of EV CRISPRa (top) and KIF11 CRISPRa (bottom) cells showing highly dispersed pericentrin and erroneous chromosome segregation in activated cells with more than two pericentrin foci. Left to right: DAPI, α-tubulin, CREST, pericentrin and composite images. Yellow arrowheads mark lagging chromosomes and extra pericentrin foci. (F) Percentage of anaphase cells with more than two pericentrin foci in EV CRISPRa and KIF11 CRISPRa cells (Fisher's exact test, n =330 and 317 cells for EV CRISPRa and KIF11 CRISPRa , respectively, three replicates). (G,H) Percentage of anaphase cells with CREST-positive (centric) lagging chromosomes (G) and CREST-negative (acentric) lagging chromosomes (H) in EV CRISPRa and KIF11 CRISPRa cells (Fisher's exact test). (I) Quantification (left) of acentric and centric micronuclei in EV CRISPRa and KIF11 CRISPRa cells (Fisher's exact test) with example composite images (right) of nuclei stained with DAPI and centromeres stained with CREST. The lower panels are magnified views of micronuclei. Data show the mean±s.e.m. * P <0.05; *** P <0.0005. Scale bars: 10 μm (A); 12 μm (E); 6 μm (I, top); 2 μm (I, bottom).
Crest Autoimmune Serum Antibody, supplied by Antibodies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Tau loss alters genetic integrity of rDNA and CDA −/− cells. a , b Representative immunofluorescence ( IF ) z ‐projection images showing paired Tau foci ( green ) linked by PICH-positive UFBs in BS ( a ) and HeLa ( b ) anaphase cells. Scale bar : 5 µm. DNA was visualized by DAPI staining ( white ). Centromeres were stained with CREST serum ( blue ) and UFBs were stained with PICH antibody ( red ). In the enlarged images, Tau foci at the extremities of UFBs are indicated by yellow arrows. c Mean number of total and Tau-positive UFBs per anaphase cell. d , f Mean number of R-UFBs per anaphase cell in untreated cell lines. e Relative number of R-UFBs per anaphase cell in BS-Ctrl (BLM) and BS-BLM cells left untreated or treated with 100 µM tetrahydrouridine ( THU ) for 2 × 48 h or 1 mM of deoxycytidine ( dC ) for 10 h. g Mean number of R-UFBs per anaphase cell in BS-Ctrl (BLM) and BS-BLM cells left untreated or treated with 0.4 µM aphidicolin ( APH ) or 1 µM ICRF-159 for 24 h. The results were normalized against those for control conditions (mock), which were set to 1. h HeLa-Ctrl (Tau) and HeLa-shTau cells were first transfected with either non-targeting or CDA siRNA. After 48 h, cells were again transfected with the indicated siRNAs. Three days after the second round of transfection, cells were collected for western blotting, with GAPDH as the loading control ( h ), or were immunostained with antibody against γ-H2AX or 53BP1 ( i , j ). At least 1500 cells were acquired by wide-field microscopy, and γ-H2AX ( i ) or 53BP1 ( j ) foci were counted. The number of cells ( n ) for each condition is indicated. k , l Cells were immunostained with antibodies against PICH and UBTF, and the mean numbers of total and rDNA-associated UFBs per anaphase cell were monitored. For IF experiments, DNA was counterstained with DAPI. For UFB counting, at least 200 cells were acquired by wide-field microscopy. For all data bars, error bars represent ± SD from at least three independent experiments. The significance of differences was assessed in two-tailed unpaired Student’s t -tests. *** P < 0.0005, ** P < 0.005, * P < 0.05, NS not significant

Journal: Nature Communications

Article Title: A role for Tau protein in maintaining ribosomal DNA stability and cytidine deaminase-deficient cell survival

doi: 10.1038/s41467-017-00633-1

Figure Lengend Snippet: Tau loss alters genetic integrity of rDNA and CDA −/− cells. a , b Representative immunofluorescence ( IF ) z ‐projection images showing paired Tau foci ( green ) linked by PICH-positive UFBs in BS ( a ) and HeLa ( b ) anaphase cells. Scale bar : 5 µm. DNA was visualized by DAPI staining ( white ). Centromeres were stained with CREST serum ( blue ) and UFBs were stained with PICH antibody ( red ). In the enlarged images, Tau foci at the extremities of UFBs are indicated by yellow arrows. c Mean number of total and Tau-positive UFBs per anaphase cell. d , f Mean number of R-UFBs per anaphase cell in untreated cell lines. e Relative number of R-UFBs per anaphase cell in BS-Ctrl (BLM) and BS-BLM cells left untreated or treated with 100 µM tetrahydrouridine ( THU ) for 2 × 48 h or 1 mM of deoxycytidine ( dC ) for 10 h. g Mean number of R-UFBs per anaphase cell in BS-Ctrl (BLM) and BS-BLM cells left untreated or treated with 0.4 µM aphidicolin ( APH ) or 1 µM ICRF-159 for 24 h. The results were normalized against those for control conditions (mock), which were set to 1. h HeLa-Ctrl (Tau) and HeLa-shTau cells were first transfected with either non-targeting or CDA siRNA. After 48 h, cells were again transfected with the indicated siRNAs. Three days after the second round of transfection, cells were collected for western blotting, with GAPDH as the loading control ( h ), or were immunostained with antibody against γ-H2AX or 53BP1 ( i , j ). At least 1500 cells were acquired by wide-field microscopy, and γ-H2AX ( i ) or 53BP1 ( j ) foci were counted. The number of cells ( n ) for each condition is indicated. k , l Cells were immunostained with antibodies against PICH and UBTF, and the mean numbers of total and rDNA-associated UFBs per anaphase cell were monitored. For IF experiments, DNA was counterstained with DAPI. For UFB counting, at least 200 cells were acquired by wide-field microscopy. For all data bars, error bars represent ± SD from at least three independent experiments. The significance of differences was assessed in two-tailed unpaired Student’s t -tests. *** P < 0.0005, ** P < 0.005, * P < 0.05, NS not significant

Article Snippet: The primary antibodies and dilutions used were: mouse anti-Tau-1 (PC1C6, #MAB3420, Millipore, dilution 1:500); rabbit anti-Tau (phospho Thr231) (#ab195739, Abcam, dilution 1:500); rabbit anti-Tau (phosphor Ser396) (#ab109390, Abcam, dilution 1:1000); rabbit anti-UBTF (H-300, #sc-9131, Santa Cruz, dilution 1:100); mouse anti-UBTF (F-9, #sc-13125, Santa Cruz, dilution 1:100); rabbit anti-PICH (#H00054821-D01P, Abnova, dilution 1:150); mouse anti-PICH (3F12-2B10, #H00054821-M01, Abnova, dilution 1:100); rabbit anti-FANCD2 (#NB100-182, Novus Biologicals, dilution 1:200); human anti-CREST (#15-234-0001, Antibodies Online GmbH, dilution 1:100); rabbit anti-γH2AX (#39117, Active Motif, dilution 1:500); mouse anti-53BP1 (clone BP13, #MAB3802, Millipore, dilution 1:500).

Techniques: Immunofluorescence, Staining, Transfection, Western Blot, Microscopy, Two Tailed Test

Increased KIF11 causes PCM fragmentation and unaligned and lagging chromosomes, which give rise to increased numbers of micronuclei. (A) Images of EV CRISPRa (top) and KIF11 CRISPRa (bottom) cells showing dispersed pericentrin and erroneous chromosome congression upon activation. Left to right: DAPI, α-tubulin, CREST, pericentrin and composite images. Yellow arrowheads mark unaligned chromosomes and extra pericentrin foci. (B) Percentage of metaphase cells with more than two pericentrin foci in EV CRISPRa and KIF11 CRISPRa cells (Fisher's exact test). (C) Percentage of metaphase cells with unaligned chromosomes in EV CRISPRa and KIF11 CRISPRa cells (Fisher's exact test). (D) Percentage of metaphase cells with unaligned chromosomes, grouped by pericentrin foci count of two (red) or greater than two (blue) in EV CRISPRa and KIF11 CRISPRa cells (Fisher's exact test). For B–D, n =315 and 324 cells for EV CRISPRa and KIF11 CRISPRa , respectively, three replicates. (E) Images of EV CRISPRa (top) and KIF11 CRISPRa (bottom) cells showing highly dispersed pericentrin and erroneous chromosome segregation in activated cells with more than two pericentrin foci. Left to right: DAPI, α-tubulin, CREST, pericentrin and composite images. Yellow arrowheads mark lagging chromosomes and extra pericentrin foci. (F) Percentage of anaphase cells with more than two pericentrin foci in EV CRISPRa and KIF11 CRISPRa cells (Fisher's exact test, n =330 and 317 cells for EV CRISPRa and KIF11 CRISPRa , respectively, three replicates). (G,H) Percentage of anaphase cells with CREST-positive (centric) lagging chromosomes (G) and CREST-negative (acentric) lagging chromosomes (H) in EV CRISPRa and KIF11 CRISPRa cells (Fisher's exact test). (I) Quantification (left) of acentric and centric micronuclei in EV CRISPRa and KIF11 CRISPRa cells (Fisher's exact test) with example composite images (right) of nuclei stained with DAPI and centromeres stained with CREST. The lower panels are magnified views of micronuclei. Data show the mean±s.e.m. * P <0.05; *** P <0.0005. Scale bars: 10 μm (A); 12 μm (E); 6 μm (I, top); 2 μm (I, bottom).

Journal: Journal of Cell Science

Article Title: Modest increase of KIF11 expression exposes fragilities in the mitotic spindle, causing chromosomal instability

doi: 10.1242/jcs.260031

Figure Lengend Snippet: Increased KIF11 causes PCM fragmentation and unaligned and lagging chromosomes, which give rise to increased numbers of micronuclei. (A) Images of EV CRISPRa (top) and KIF11 CRISPRa (bottom) cells showing dispersed pericentrin and erroneous chromosome congression upon activation. Left to right: DAPI, α-tubulin, CREST, pericentrin and composite images. Yellow arrowheads mark unaligned chromosomes and extra pericentrin foci. (B) Percentage of metaphase cells with more than two pericentrin foci in EV CRISPRa and KIF11 CRISPRa cells (Fisher's exact test). (C) Percentage of metaphase cells with unaligned chromosomes in EV CRISPRa and KIF11 CRISPRa cells (Fisher's exact test). (D) Percentage of metaphase cells with unaligned chromosomes, grouped by pericentrin foci count of two (red) or greater than two (blue) in EV CRISPRa and KIF11 CRISPRa cells (Fisher's exact test). For B–D, n =315 and 324 cells for EV CRISPRa and KIF11 CRISPRa , respectively, three replicates. (E) Images of EV CRISPRa (top) and KIF11 CRISPRa (bottom) cells showing highly dispersed pericentrin and erroneous chromosome segregation in activated cells with more than two pericentrin foci. Left to right: DAPI, α-tubulin, CREST, pericentrin and composite images. Yellow arrowheads mark lagging chromosomes and extra pericentrin foci. (F) Percentage of anaphase cells with more than two pericentrin foci in EV CRISPRa and KIF11 CRISPRa cells (Fisher's exact test, n =330 and 317 cells for EV CRISPRa and KIF11 CRISPRa , respectively, three replicates). (G,H) Percentage of anaphase cells with CREST-positive (centric) lagging chromosomes (G) and CREST-negative (acentric) lagging chromosomes (H) in EV CRISPRa and KIF11 CRISPRa cells (Fisher's exact test). (I) Quantification (left) of acentric and centric micronuclei in EV CRISPRa and KIF11 CRISPRa cells (Fisher's exact test) with example composite images (right) of nuclei stained with DAPI and centromeres stained with CREST. The lower panels are magnified views of micronuclei. Data show the mean±s.e.m. * P <0.05; *** P <0.0005. Scale bars: 10 μm (A); 12 μm (E); 6 μm (I, top); 2 μm (I, bottom).

Article Snippet: Antibodies were used as follows: mouse α-tubulin (Thermo Fisher Scientific, 32-2500), 1:5000 (western blotting or WB) and 1:500 (immunofluorescence or IF); rabbit α-tubulin (Abcam, ab52866), 1:500 (IF); mouse CENP-A (Abcam, ab13939), 1:200 (IF); human CREST (Antibodies Incorporated, 15-234-0001), 1:300 (IF); rabbit pericentrin (Abcam, ab4448), 1:2000 (IF); rabbit KIF11 (Novus Biologicals, NB5000-181), 1:1000 (IF); mouse p53 (Cell Signaling Technologies, 48818S), 1:1000 (IF); and rabbit MAD2 (Millipore UK, MABE866), 1:1000 (WB) and 1:500 (IF).

Techniques: Activation Assay, Staining